A reverse phase high performance liquid chromatographic method (RP-HPLC) has been developed for the simultaneous estimation of Paracetamol (PARA), Aceclofenac (ACE) and Tizanidine (TIZ) in the pharmaceutical formulation using RP-C18 (250 mm x 4.6 mm, 5 μm) column used as stationary phase. The mobile phase (Acetonitrile, Methanol and Orthophosphoric acid pH.3.5 in HPLC grade water) was pumped at a flow rate of 1.3 ml/min in the ratio of 50:40:10 and the eluents were monitored at 260.0 nm. Linearity was obtained in the concentration range of 100-1500μg/ml for PARA, 1-500μg/ml ACE and Tizanidine hydrochloride having range of 1-20 μg/ml. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed RP-HPLC method may be used for determining Paracetamol, Aceclofenac and Tizanidine in bulk drug samples.
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